PhD student in immunology
Open position for PhD student in Immunology. The accumulation of critical receptors at the tips of membrane protrusions of unstimulated B and T cells was recently discovered by our group and others (refs.). Such nanoscopic organisation of receptors is key for regulation of signalling events controlling complex immune responses in mammals. In this project, we will study how stress factors (e.g., temperature or oxidative burst) affect three-dimensional organisation of receptors (TCR, CD2, CD4, CD5, CD6) on T-cell surface and their associated effector molecules (e.g., kinases and adaptor molecules) before interaction with stimulating targets. This research will help to understand responsivity/function of T cells in the inflammatory sites, which are often referred to as crucial sites linked to the pathology of autoimmune diseases.
The work will include imaging of receptors and associated effector molecules on the surface of stressed T cells. The imaging approaches will include a panel of advanced fluorescence and super-resolution microscopy techniques, which will be complemented by flow cytometry and some basic biochemistry methods to determine the impact of stress on T-cell function.
Requirements
- Master Degree in Biology, Master degree in Immunology
- Handling of lymphocytes, recombinant DNA techniques, flow cytometry and spectrophotometry
- Statistical analysis for biomedicine
- Experience with handling organic solvents and compounds
- Experience with project coordination
- Prism - GraphPad experience
- Programming in R.
Eligibility criteria
To apply, please send your CV and motivation letter to recruitment
jh-inst.cas.cz. Please, write in the subject line of your email SC2022_28.
Benefits
- 25 days paid leave of absence (holidays) per year + 3 days of paid sick-leave in addition to the statutory insurance
- Flexible working-time, the possibility of part-time work, work-life balance
- Lunch vouchers
- Subsidy for sports and cultural activities
- Recreation subsidy, recreation subsidy for children
- Possibility of attending children groups of the CAS
- Possibility to use discounts in recreation centers of the CAS
- Sports goods rental, free parking, trade union library
- Language courses
- Accommodation facilities
- Attractive location
- Health and social insurance paid mandatory by the institute
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