PhD. student in biophysics/physical chemistry
The position is part of the Innovative Training Network (ITN) "PROTON". The network is devoted to the investigation of proton transport and proton-coupled transport. It is funded under Marie Sklodowska-Curie actions (H2020-MSCA-ITN-2019, grant agreement number: 860592). The successful applicant will focus on the natural voltage-gated proton channel Hv1 and investigate the protons pathway in the channel using time dependent fluorescence shift measurements and FRET.
The Ph.D. student will have two secondments:
• Johannes Kepler University, Linz, Austria (2 months): measurements of unitary channel conductance in reconstituted
GUVs
• INTANA, Germany (2 months): FCCS of Hv1 and antennae inhibiting elements
Johannes Kepler University, Linz, Austria (2 months): electrophysiological measurements of proton conductivity using planar lipid bilayer.
Finances
The applicant will be funded from the EU project for the 1st 19 months, but not later as till the project termination in July 2024. After that, a successful Ph.D. student will be prolonged for additional 17 months and will be funded from a different project based on the Czech funding practice.
Additional information
Women are encouraged to apply. The recruitment committee gives preference to female candidates if they are equally qualified as male candidates.
Type of contract: Temporary
Job status: Part-time
Offer starting date: November 2022
Required Qualifications
1. Applicants must, at the date of recruitment by PROTON, be in the first four years (full-time equivalent research experience measured from the date when the researcher obtained the degree entitling him/her to embark on a doctorate) of their research careers.
2. They should not have been awarded a doctoral degree.
3. Applicants must not have resided or carried out their main activity (work, studies, etc.) in the country of the recruiting institution for more than 12 months in the 3 years immediately before the recruitment date. This excludes short stays such as holidays or compulsory national service.
4. We look for students with exceptional academic performance holding or very close to completing their Master degree in Biophysics, Biochemistry or closely related subjects.
5. Knowledge of the English language is essential.
Additional Qualifications
Advanced methods of fluorescence spectroscopy and microscopy. Basic preparation skills of the model bilayer systems and protein labelling. Biochemical labelling, chromatography, fluorescence spectroscopy, time resolved anisotropy and circular dichroism spectroscopy, single molecule microscopy.
1. Knowledge of fluorescence spectroscopy and microscopy techniques (e.g. confocal microscopy, fluorescence correlation spectroscopy, TCSPC)
2. Experience in handling fluorescent molecules, etc.
3. Publications in international journals/attendance of international conferences in the following fields: biophysics, fluorescence spectroscopy.
4. Protein purification and membrane reconstitution
5. Ability to use programming languages for data acquisition
6. Soft skills: presentation skill, team working ability
Requeired languages
English: Good
For all the above, knowledge is proven by publications, certificates, master thesis or equivalent documentation. Skills should be acknowledged in reference letters and demonstrated during the interview (presentation skills). During the interview each applicant presents his/her master thesis and a scientific paper from an area unrelated to his/her specialization (chosen by the interviewers, approximately 5 minutes).
Benefits
- 25 days paid leave of absence (holidays) per year + 3 days of paid sick-leave in addition to the statutory insurance
- Flexible working-time, the possibility of part-time work, work-life balance
- Lunch vouchers
- Subsidy for sport and cultural activities
- Recreation subsidy, recreation subsidy for children
- Possibility of attending children groups of the CAS
- Possibility to use discounts in recreation centers of the CAS
- Sports goods rental, free parking, trade union library
- Language courses
- Accommodation facilities
Eligibility criteria
To apply, please send the required documents to recruitment
jh-inst.cas.cz and jan.sykorajh-inst.cas.cz. Please, write in the subject line of your email SC2022_24.
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