Interaction of antibacterial peptides with the model membranes and possibility of the prediction of their biological activity
Grant Agency of the Czech Republic
Abstract: A set of experimental techniques, like circular dichroism (CD), stopped flow CD, FTIR, Raman spectroscopy, and fluorescence spectroscopy including fluorescence correlation spectroscopy will be employed to study the newly isolated antibacterial peptides (AMP) from bees' venom and their synthetic analogs. Mechanism of AMPs action will be investigated by means of interaction with the selected model membranes mimicking different types of cells. The proposed model systems in combination with the selected peptides and the used experimental methods enable understanding mode of action of AMPs. Correlation of the results with biological tests will be helpful for the design of the peptide analogues synthesized for medical purpose.