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The labelling of proteins by an organometallic marker
Our study involved the complicated reduction mechanism of two organometallic
compounds cymantrene and its ring substituted derivative cymantrenyl methyl imidate,
which we had chosen for labelling of bioorganic
macromolecules (BSA). One-electron
reduction yields very unstable 19-electron intermediate which undergoes further
chemical reactions. Comparison of the data obtained under atmosphere of argon and
carbon monoxide led to the conclusion that a substitution of ligand CO takes part in
reaction. The electrochemical method for detection of proteins labelled by redox active
organometallic compound is important for immunoassay analysis.
publications 1999-2003