Understanding, control, and preparation of self-assembling biomembrane systems at interfaces.
No. 203/05/2308, Grant Agency of the Czech Republic
In 1984 it was discovered that the exposure of a solid support to phospholipid vesicle suspensions may lead to the formation of supported phospholipid bilayers (SPBÂ´s). Though SPBÂ´s have now been applied in biosensors, blood-compatible surfaces, medical implant devices, and production of catalytic interfaces, a consistent picture on SPB formation and the parameters influencing the mechanisms and properties of the resulting adsorbed layers are still missing. This proposal aims for a consistent picture onSPB formation by applying five complementary techniques (fluorescence correlation spectroscopy (FCS), time-resolved single molecule fluorescence, ellipsometry, atomic force microscopy, electron spectroscopy for chemical analysis; all available at the JH-Inst) and using a broad choice of materials (supports, lipids, buffers, cholesterol, proteins, probes) and conditions (surface treatment, vesicle preparation, stacking conditions).
prof. HOF Martin, Dr. rer. nat. DSc.
Department: Department of Biophysical Chemistry
Phone: (+420) 26605 3264, 2011
Fax: (+420) 266053910
Room: 221,107 c